innuTaq DNA Polymerase

Thermostable enzyme for routine PCR.

  • Optimal for routine PCR
  • Convenient and reliable amplification
  • Extremely thermostable
  • Best reproducible results

Product Details

Analytik Jena‘s innuTaq DNA Polymerase is a highly purified recombinant thermostable DNA polymerase that has been isolated from E.coli carrying a vector encoding the Thermus aquaticus DNA polymerase gene. The enzyme has 5’ → 3’ DNA polymerase activity. It exhibits high thermal stability in withstanding prolonged incubations at elevated temperatures (95 °C). It is recommended for use in routine PCR reactions.

Specifications

Concentration

5 U/µl (One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C)

PCR Buffer

  • 10× PCR Buffer with KCl; 100 mM Tris-HCl (pH 8.8 at 25 °C); 500 mM KCl; 0.8 % Nonidet P40
  • 10× PCR Buffer with (NH4)2SO4; 750 mM Tris-HCl (pH 8.8 at 25 °C); 200 mM (NH4)2SO4; 0.1 % Tween 20

Enzyme storage buffer

20 mM Tris-HCl pH 8.0; 100 mM KCl; 1 mM DTT; 0.1 mM EDTA; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % Glycerol

PS-Mg2+ solution

MgCl2 stock solution, 25 mM

Ask about the products

Sed arcu lectus auctor vitae, consectetuer et venenatis eget velit. Sed augue orci, lacinia eu tincidunt et eleifend nec lacus. Donec ultricies nisl ut felis, suspendisse potenti. This can be edited in here when logged in.

Contact request

  • This field is for validation purposes and should be left unchanged.